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This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includestime-tested coremethods and new innovationsapplicable to any researcher * Methods included are useful to both establishedresearchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

Historical perspective
3D reconstruction from electron micrographs: a personal account of its development
David DeRosier
Preparation of 2-D crystals
Henning Stahlberg
Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie
Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen
A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski
GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark
Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark
Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang
Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky
Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld
How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun
Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng
Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat
Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz
Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama
Radiation damage in electron cryomicroscopy
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