Amyloid precursor protein (APP) is an extensively studied transmembrane protein, and its derivative, amyloid b-protein, has been implicated as the primary cause of Alzheimer's disease. Thousands of reports have been published on APP, and interest continues to grow in the research community. In the search for an effective treatment for Alzheimer's disease, APP is a unique model protein that illustrates the wide array of basic and sophisticated techniques available for characterization. "Amyloid Precursor Protein: A Practical Approach" presents a variety of biological techniques utilized in genetics, molecular biology, cell biology, and biochemistry to elucidate the structure and function of this transmembrane protein. Each technique is presented with detailed, step-by-step protocols to achieve reproducible results and provide a framework for studying other membrane proteins. These protocols also form the core of the authors' approaches to elucidating the function of APP. This timely book contrasts basic and advanced methods to explain how the development of biological techniques affects the way molecular targets are examined. Practical in scope, it provides useful lists of protocols and routinely used antibodies for easy reference and paints a complete picture of the current understanding of APP as well as direction for future research.
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