1. Introduction to Fluorescence.
2. Instrumentation for Fluorescence Spectroscopy.
3. Fluorophores.
4. Time-Domain Lifetime Measurements.
5. Frequency-Domain Lifetime Measurements.
6. Solvent Effects on Emission Spectra.
7. Dynamics of Solvent and Spectral Relaxation.
8. Quenching of Fluorescence.
9. Advanced Topics in Fluorescence Quenching.
10. Fluorescence Anistropy.
11. Time-Dependent Anisotropy Decays.
12. Advanced Anisotropy Concepts.
13. Energy Transfer.
14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers.
15. Energy Transfer to Multiple Acceptors in One, Two or Three-Dimensions.
16. Protein Fluorescence.
17. Time-Resolved Protein Fluorescence.
18. Excited State Reactions.
19. Fluorescence Sensing
20. Long Lifetime Metal-Ligand Complexes.
21. DNA Technology.
22. Phase Sensitive and Phase Resolved Emission Spectra.
Appendixes: I. Corrected Emission Spectra.
II. Fluorescent Lifetime Standards.
III. Additional Reading Index
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